Dr. Yebra has extensive experience working on carbohydrate metabolism, regulation and molecular genetics of lactic acid bacteria. In the last few years she is interested in elucidating the role of human milk oligosaccharides (HMOs) in infant gut bacterial colonization. Human milk contains a large number of oligosaccharides and much evidence suggests that they are associated with the protective effect of human milk against gastroenteritis through its prebiotic effect, thus stimulating beneficial bacteria growth in the colon, but also through competitive inhibition of binding (anti-adhesion) of entero-pathogens to the host cells. The general objectives of the lab are (i) the molecular and functional characterization of the HMOs metabolic pathways in lactic acid bacteria; (ii) evaluation of the HMOs prebiotic activity and (iii) the analysis of the blocking activity exerted by specific HMOs into the pathogens attachment to their receptors. Since oligosaccharides are abundant in human milk but not in infant formula, we are also interested in the identification, and molecular and functional analysis of glycosidases to synthesize HMOs-like through their transglycosylation activity. Another research area of the lab is the development of metabolic engineering strategies for the production of biotechnological important metabolites in lactic acid bacteria.
B.S. Biology, University of Oviedo, Spain, 1986
Ph.D. Biology, University of Oviedo, Spain, 1991
SCIENTIFIC AND PROFESSIONAL ACTIVITIES
Postdoctoral position, Wayne State University, Detroit, USA, 1992-1994
Research Staff, IATA-CSIC, Valencia, Spain, since 1995
Rubio-del-Campo A, Gozalbo-Rovira R, Moya-Gonzálvez EM, Alberola J., Rodríguez-Díaz J. and Yebra MJ. 2021. Infant gut microbiota modulation by human milk disaccharides in humanized microbiome mice. Gut Microbes; VOL 13, NO. 1, 1-20 https://doi.org/10.1080/19490976.2021.1914377.
Becerra J.E., Rodríguez-Díaz J., Gozalbo-Rovira R., Palomino-Schätzlein M., Zúñiga M., Monedero V. and Yebra, M.J. 2020. Unique microbial catabolic pathway for the human-core N-glycan constituent fucosyl-alpha-1,6-N-acetylglucosamine-asparagine. mBio 11: e02804-19. https://doi.org/10.1128/mBio.02804-19
Bidart N.G., Rodríguez-Díaz J., Pérez-Martínez G. and Yebra, M.J. 2018. The lactose operon from Lactobacillus casei is involved in the transport and metabolism of the human milk oligosaccharide core-2 N-acetyllactosamine. Scientific Reports 8:7152
Bidart N.G., Rodríguez-Díaz J., Palomino-Schätzlein M., Monedero V. and Yebra, M.J. 2017. Human milk and mucosal lacto- and galacto-N-biose synthesis by transgalactosylation and their prebiotic potential in Lactobacillus species. Appl Microbiol Biotechnol 101:205-215.
Bidart N.G., Rodríguez-Díaz J. and Yebra, M.J. 2016. The extracellular wall-bound beta-N-acetylglucosaminidase from Lactobacillus casei is involved in the metabolism of the human milk oligosaccharide lacto-N-triose. Appl Environ Microbiol 82:570-577
Becerra J.E., Coll-Marqués J.M., Rodríguez-Díaz J., Monedero V. and Yebra, M.J. 2015. Preparative scale purification of fucosyl-N-acetylglucosamine disaccharides and their evaluation as potential prebiotics and anti-adhesins. Appl Microbiol Biotechnol 99:7165-7176
- Nº Solicitud:P201431600Título:Beta-D-transgalactosidasa de Lactobacillus caseiFecha de solicitud:divendres, 31 octubre, 2014
- Nº Solicitud:N201030438Título:Truncated phytases from Bifidobacterium and their usesFecha de solicitud:dimecres, 24 març, 2010