Iron is an essential micronutrient for all eukaryotes. However, iron deficiency anemia is the most extended nutritional disorder worldwide. Thus, our main objective consists on deciphering the molecular mechanisms that eukaryotic cells utilize to regulate the response to iron deficiency. For this purpose, we use the yeast Saccharomyces cerevisiae as a model organism and biotechnological tool, paying special attention to transcriptional, post-transcriptional and metabolic regulation strategies. We use a multidisciplinary approach that includes Molecular Biology, Biochemistry, Microbiology, Genetics, Cellular Biology and "Omic" techniques.
Caracterización y utilización biotecnológica de factores reguladores de la respuesta a la deficiencia de hierro en levadura y plantas
AGL2011-29099
Caracterización y utilización de mecanismos globales para la obtención y uso alimentario de levaduras industriales enriquecidas en hierro
Publications
Sanvisens N, Romero AM, An X, Zhang C, de Llanos R, Martínez-Pastor MT, Bañó MC, Huang M, Puig S (2014). Yeast Dun1 kinase regulates ribonucleotide reductase inhibitor Sml1 in response to iron deficiency. Mol. Cell. Biol. 34 (17): 3259-3271. http://www.ncbi.nlm.nih.gov/pubmed/24958100
Martínez-Pastor M, Vergara SV, Puig S, Thiele DJ (2013). Negative feedback regulation of the yeast Cth1 and Cth2 mRNA binding proteins is required for adaptation to iron deficiency and iron supplementation. Mol Cell Biol. 33(11): 2178-2187. http://www.ncbi.nlm.nih.gov/pubmed/23530061
Sanvisens N, Bañó MC, Huang M, and Puig S. (2011). Regulation of ribonucleotide reductase in response to iron deficiency. Molecular Cell. 44(5): 759-769. **Premio Fisher Scientific otorgado por la Sociedad Española de Bioquímica y Biología Molecular (SEBBM) al mejor trabajo de investigación publicado en España por un socio menor de 31 años. http://www.ncbi.nlm.nih.gov/pubmed/22152479
Puig S, Vergara SV and Thiele DJ (2008). Cooperation of two mRNA-binding proteins drives metabolic adaptation to iron deficiency. Cell Metabolism. 7(6): 555-564. http://www.ncbi.nlm.nih.gov/pubmed/18522836
Puig S, Askeland E, Thiele DJ. (2005). Coordinated remodeling of cellular metabolism during iron deficiency through targeted mRNA degradation. Cell. 120(1): 99-110. http://www.ncbi.nlm.nih.gov/pubmed/15652485
