I obtained my PhD in Biochemistry and Molecular Biology by the University of Valencia with the thesis entitled "Development of monoclonal antibody-based immunoassays for the determination of the insecticide azinphos-methyl in environmental waters and in fruit juices". Subsequently, I carried out a two-year postdoctoral stage at the Technical University of Munich, thanks to a Marie Curie Research Grant, with the aim of generating recombinant molecular receptors specific of small organic chemicals. Afterwards, I was engaged with private research during several years; first, in Lyon, at the company BioMérieux where I studied innovative analytical strategies applied to the microbiological safety of food, and then, in Barcelona, at the Food Diagnostics division of UCB Pharma where I developed commercial tests for the detection of veterinary drugs in milk.
Nowadays, I am a member of the Food Analytical Immunotechnology group located at the Institute of Agrochemistry and Food Technology (IATA), which belongs to the Spanish National Research Council (CSIC) - the largest public R&D institution in Spain. Our research is focused on practical and basic subjects concerning the generation of high-quality functionalized haptens, conjugates, and bioreceptors of chemical residues and contaminants that are potentially present in food. A relevant collection of derivatives of analytical targets as well as polyclonal and monoclonal antibodies has been raised and is being kept at the IATA. Moreover, novel rapid methods have been developed and validated for the analysis of a significant number of toxic xenobiotics in a wide variety of foodstuffs.
López-Puertollano D, Agulló C, MERCADER JV, Abad-Somovilla A, Abad-Fuentes A. Immunoanalytical methods for ochratoxin A monitoring in wine and must based on innovative immunoreagents. Food Chemistry 2021, 345, 128828. DOI: 10.1016/j.foodchem.2020.128828.
Ceballos-Alcantarilla E, Abad-Fuentes A, Agulló C, Abad-Somovilla A, MERCADER JV. Immunochemical method for penthiopyrad detection through thermodynamic and kinetic characterization of monoclonal antibodies. Talanta 2021, 226, 122123. DOI: 10.1016/j.talanta.2021.122123.
Cevallos-Cedeño RE, Agulló C, Abad-Fuentes A, Abad-Somovilla A, MERCADER JV. Enzyme and lateral flow monoclonal antibody-based immunoassays to simultaneously determine spirotetramat and spirotetramat-enol in foodstuffs. Scientific Reports 2021, 11, 1809. DOI: 10.1038/s41598-021-81432-z.
Cavalera S, Agulló C, MERCADER JV, Di Nardo F, Chiarello M, Anfossi L, Baggiani C, D’Avolio A, Abad-Somovilla A, Abad-Fuentes A. Monoclonal antibodies with subnanomolar affinity to tenofovir for monitoring adherence to antiretroviral therapies: from hapten synthesis to prototype development. Journal of Materials Chemistry B 2021, 8, 10439‒10449. DOI: 10.1039/D0TB01791D.
López-Puertollano D, Agulló C, MERCADER JV, Abad-Somovilla A, Abad-Fuentes A. Click chemistry-assisted bioconjugates for hapten immunodiagnostics. Bioconjugate Chemistry 2020, 31, 956‒964. DOI: 10.1021/acs.bioconjchem.0c00099.
Pérez-Fernández B, MERCADER JV, Abad-Fuentes A, Checa-Orrego BI, Costa-García A, de la Escosura-Muñiz A. Direct competitive immunosensor for imidacloprid pesticide detection on gold nanoparticle-modified electrodes. Talanta 2020, 209, 120465. DOI: 10.1016/j.talanta.2019.120465.
Lafon PA, Wang Y, Arango-Lievano M, Torrent J, Salvador-Prince L, Mansuy M, Mestre-Francès N, Givalois L, Liu J, MERCADER JV, Jeanneteau F, Desrumaux C, Perrier V. Fungicide residues exposure and β-amyloid aggregation in a mouse model of Alzheimer’s disease. Environmental Health Perspectives 2020, 128, 017011. DOI: 10.1289/EHP5550.
MERCADER JV, Agulló C, Abad-Somovilla A, Abad-Fuentes A. A monoclonal antibody-based immunoassay for mepanipyrim residue sensitive analysis in grape juice and wine. Food Analytical Methods 2020, 13, 770‒779. DOI: 10.1007/s12161-019-01693-3.
Quiñones-Reyes G, Agulló C, MERCADER JV, Abad-Somovilla A, Abad-Fuentes A. Synthetic haptens and monoclonal antibodies to the cyanotoxin anatoxin-a. Angewandte Chimie International Edition 2019, 58, 9134‒9139. DOI: 10.1002/anie.201904002.
Pérez-Fernández B, MERCADER JV, Checa-Orrego BI, de Escosura-Muñiz A, Costa-García A. Monoclonal antibody-based immunosensor for the electrochemical detection of imidacloprid pesticide. Analyst 2019, 144, 2936‒2941. DOI: 10.1039/c9an00176j.
Ceballos-Alcantarilla E, Agulló C, Abad-Somovilla A, Abad-Fuentes A, MERCADER JV. Highly sensitive monoclonal antibody-based immunoassays for the analysis of fluopyram in food samples. Food Chemistry 2019, 288, 117‒126. DOI: 10.1016/j.foodchem.2019.03.007.
Ceballos-Alcantarilla E, López-Puertollano D, Agulló C, Abad-Fuentes A, Abad-Somovilla A, MERCADER JV. Combined heterologies for monoclonal antibody-based immunoanalysis of fluxapyroxad. Analyst 2018, 143, 5718‒5727. DOI: 10.1039/C8AN01771A.
Cevallos-Cedeño RE, Agulló C, Abad-Somovilla A, Abad-Fuentes A, MERCADER JV. Hapten design and antibody generation for immunoanalysis of spirotetramat and spirotetramat-enol. ACS Omega 2018, 3, 11950‒11957. DOI: 10.1021/acsomega.8b01784.
Duncan H, Abad-Somovilla A, Abad-Fuentes A, Agulló C, MERCADER JV. Immunochemical rapid determination of quinoxyfen, a priority hazardous pollutant. Chemosphere 2018, 211, 302‒307. DOI: 10.1016/j.chemosphere.2018.07.166.
Esteve-Turrillas FA, Agulló C, MERCADER JV, Abad-Somovilla A, Abad-Fuentes A. Rationally designed haptens for highly sensitive monoclonal antibody-based immunoanalysis of fenhexamid. Analyst 2018, 143, 4057‒4066. DOI: 10.1039/C8AN00827B.
Orellana-Silla A, Armenta S, de la Guardia M, MERCADER JV, Esteve-Turrillas FA. Development of immunosorbents for the analysis of forchlorfenuron in fruit juices by ion mobility spectrometry. Analytical & Bioanalytical Chemistry 2018, 410, 5961‒5967. DOI: 10.1007/s00216-018-1213-5.
López-Puertollano D, MERCADER JV, Agulló C, Abad-Somovilla A, Abad-Fuentes A. Novel haptens and monoclonal antibodies with subnanomolar affinity for a classical analytical target, ochratoxin A. Scientific Reports 2018, 8, 1‒9. DOI: 10.1038/s41598-018-28138-x.
Esteve-Turrillas FA, MERCADER JV, Agulló C, Abad-Somovilla A, Abad-Fuentes A. Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries. Food Chemistry 2018, 267, 2‒9. DOI: 10.1016/j.foodchem.2017.06.013.
Addante-Moya LG, Agulló C, Quiñones-Reyes G, MERCADER JV, Abad-Fuentes A, Abad-Somovilla A. A unified approach to the synthesis of both enantiomers of anatoxin-a and homoanatoxin-a cyanotoxins. Tetrahedron 2018, 74, 5022‒5031. DOI: 10.1016/j.tet.2018.04.057.
MERCADER JV, Abad-Somovilla A, Agulló C, Abad-Fuentes A. Fluxapyroxad haptens and antibodies for highly sensitive immunoanalysis of food samples. Journal of Agricultural and Food Chemistry 2017, 65, 9333‒9341. DOI: 10.1021/acs.jafc.7b03199.
Esteve-Turrillas FA, MERCADER JV, Agulló C, Abad-Somovilla A, Abad-Fuentes A.A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten. Analyst 2017, 142, 3975‒3985. DOI: 10.1039/C7AN01138E.
Ceballos-Alcantarilla E, Agulló C, Abad-Fuentes A, Escamilla-Aguilar M, Abad-Somovilla A, MERCADER JV. High-affinity antibodies from a full penthiopyrad-mimicking hapten and heterologous immunoassay development for fruit juice analysis. Food Analytical Methods 2017, 10, 4013‒4023. DOI: 10.1007/s12161-017-0974-3.
Ceballos-Alcantarilla E, Abad-Somovilla A, Agulló C, Abad-Fuentes A, MERCADER JV. Protein-free hapten-carbon nanotube constructs induce the secondary immune response. Bioconjugate Chemistry 2017, 28, 1630‒1638. DOI: 10.1021/acs.bioconjchem.6b00653.
Kolosova A, Maximova K, Eremin SA, Zherdev AV, MERCADER JV, Abad-Fuentes A, Dzantiev BB. Fluorescence polarisation immunoassays for strobilurin fungicides kresoxim-methyl, trifloxystrobin and picoxystrobin. Talanta 2017, 162, 495‒504. DOI: 10.1016/j.talanta.2016.10.063.
- Request number:P202030897Title:Heterologous bioconjugates and their use for aflatoxin immunodetectionDate of the request:Thursday, 3 September, 2020
- Request number:P202030133Title:Bioconjugates and antibodies for derivatization-assisted immunodetection of the mycotoxin patulinDate of the request:Monday, 17 February, 2020
- Request number:P201631470Title:Preparation of novel bioconjugates and antibodies for ochratoxin A immunodetectionDate of the request:Thursday, 17 November, 2016
- Request number:P201531661Title:Preparation of bioconjugates and antibodies for the immunodetection of anatoxin-aDate of the request:Tuesday, 17 November, 2015
- Request number:P201231583Title:Functionalized derivatives and immunoreagents for the fungicide fludioxonilDate of the request:Tuesday, 16 October, 2012
- Request number:P201230098Title:Haptens, conjugates, and antibodies for the fungicide pyrimethanilDate of the request:Wednesday, 25 January, 2012
- Request number:P201131641Title:Haptens, conjugates, and antibodies for the fungicide cyprodinilDate of the request:Thursday, 13 October, 2011
- Request number:P201031671Title:Functionalized derivatives of boscalidDate of the request:Friday, 12 November, 2010
- Request number:FR03/04262Title:Method for detecting and/or identifying bacteria present in a sampleDate of the request:Monday, 7 April, 2003