Viernes 21 de mayo
Hora: 12:30h
“An engineered Calvin cycle enables the yeast Komagataella phaffii to grow and produce organic acids from CO2”, impartido por el Dr. Diethard Mattanovich.
Global warming, caused by rapidly increasing atmospheric carbon dioxide levels, calls for novel measures to sequester CO2. Assimilation of CO2 into biomass and bulk chemicals is an attractive option. Besides the use of photosynthesizing organisms, synthetic biology offers the change to develop strains for a novel CO2 based biotechnology. The methylotrophic yeast Komagataella phaffii (formerly known as Pichia pastoris) is widely used in the manufacture of industrial enzymes and pharmaceuticals. Like most biotechnological production hosts, K. phaffii is heterotrophic, growing on organic feedstocks. By addition of eight heterologous genes and deletion of three native genes, we engineered the peroxisomal methanol-assimilation pathway of K. phaffii into a CO2 fixation pathway resembling the Calvin-Benson-Bassham (CBB) cycle, the predominant natural CO2 fixation pathway. Due to the modular metabolic design which separates carbon assimilation from energy production, any NADH yielding energy source can be used. At present methanol oxidation is employed. The resulting strain can grow continuously with CO2 as a sole carbon source, demonstrating a chemoorganotrophic lifestyle. Integration of heterologous genes encoding enzymes for the synthesis of short organic acids led to the production of these chemical building blocks at the g/L level. This engineered K. phaffii strain may promote sustainability by sequestering the greenhouse gas CO2 and converting it into yeast biomass and chemicals.
El Dr. Diethard Mattanovich es Professor del Departmento de Biotecnología de la Universidad de Recursos Naturales y Ciencias de la Vida (BOKU), en Viena (Austria). Es uno de los mayores expertos mundiales en ingeniería metabólica y principal referente en la levadura modelo Pichia pastoris.
Acceso al seminario a través del siguiente link: https://us02web.zoom.us/j/81480778864?pwd=aXo4UmdQbkFiRzlxb2hwTVJjNWN6QT09
Al final de la charla se abrirá un turno de preguntas que se podrán trasladar a través del chat de la sesión o activando la cámara y el micro de quienes quieran consultar. ¡Os esperamos!